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PASC is frequently diagnosed in non-hospitalized patients with mild illness, and this population represents most COVID-19 cases 5

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doi:10.1155/2012/324832 Hussain M, et al

Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol

These aggressive protocols typically run shorter durations