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Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)
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Bagsvrd, Denmark: Novo Nordisk
Collagen production: Vitamin C can help to boost collagen production, which can help to keep the skin and mucous membranes healthy and robust
Strong J, Driscoll MS
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