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S2a, Table S1a)
Abstract Steroidal 5-reductase is a system of NADPH dependent enzyme that catalyzes the irreversible conversion of 43-ketosteroid precursor (testosterone) to its corresponding 5-reduced metabolite (dihydrotestosterone)
156 Besides, with the consumption of extracellular glutamine, GDH can also catalyze ammonia derived from glutaminolysis and -KG to support the synthesis of glutamate and downstream metabolites by reductive amination in a NADPH consumption manner to meet the cancer cells growth
For instance, vitamin B12 activates succinyl-CoA
Yes, and the combination is synergistic
The IC 50 value, which represents half the maximum concentration of a substance that inhibits a specific biological function in vitro , was determined using this assay
