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After 23 passages, LSC were plated on a fibronectin-coated flask and maintained in Iscoves Modified Dulbeccos Media (IMDM) containing 20% fetal bovine serum (FBS)

Increased mitochondrial mass and cytosolic redox imbalance in hippocampal astrocytes of a mouse model of rett syndrome: subcellular changes revealed by ratiometric imaging of JC-1 and roGFP1 fluorescence